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Immunostaing


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#1 harry348

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Posted 23 April 2014 - 03:38 AM

what is the best protocol to immunostain free floating cells?

 

Thanks in advance

 

Harry


Edited by harry348, 23 April 2014 - 03:39 AM.


#2 Tabaluga

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Posted 23 April 2014 - 08:05 AM

Can you tell us what exactly you want to stain ? Usually with suspension cells the main difference is that for the washing steps you have to resuspend in, say, PBS and then centrifuge and discard the supernatant.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#3 harry348

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Posted 21 May 2014 - 12:46 AM

Thank you very much and sorry for the late reply






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