1 reply to this topic

[connicelee]

Hi everybody,

I would like to design primer to detect some bacteria in a mixculture. I had sequenced their 16S rDNA and ITS region. However, the sequences is very similar, only a few mer different because they are from the same species. Any method that i can create a probe or primers to detect these bacteria?

Thanks a lot.

Connice Lee

[leahf]

Hi
If the few diifferent bases are in the same area, you can design specific oligonucleotides for either PCR or FISH. In my lab three different bases out of a 20 bp oligo were enough. possibly you can go lower.
Luck!