Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Collagen Gel Contraction


  • Please log in to reply
1 reply to this topic

#1 itz_jacki

itz_jacki

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 17 June 2004 - 08:24 AM

Hi,

I'm trying to use vitrogen collagen I as a stretchable substratum for seeding vascular smooth muscle cells (SMCs).

Right now, I'm using a protocol for mixing the gel which includes Vitrogen collagen stock, NaOH, and DMEM/FBS (The growth medium for my cells).

When I try stretching my collagen gel, the gel surface breaks or tears apart easily. If I want a stronger and more stretchable surface so that I can work with greater force of stretches, what should I do to make the gel stronger? Should I increase the relative proportion of Vitrogen Collagen solution?

Thanks,
Jacky

Edited by itz_jacki, 17 June 2004 - 08:27 AM.


#2 jeffreybarrett1

jeffreybarrett1

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 07 December 2004 - 09:37 AM

Collagen gel may not be the best for a stretching surface. In my experience with collagen gel, type I, I find that collagen tears very easily. It is definitely less fragile if the collagen concentration and NaOH concentration are increased, so you could try one or both of these. Sorry, I don't have any other substrate alternatives.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.