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Methanol/acetic acid fixation


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#1 Chelo

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Posted 22 April 2014 - 02:16 AM

Dear Forum members,

 

I have a basic question but I would like to know if there is any reasons why the metanol/acetic acid mix used for fixation has to be prepared at the moment of use. Isn´t it possible to keep a stock stored at -20ºC for example? Also, I am interested in fixing cells for qFISH. Is this the best choice to fix cells for telomere visualization?

Thanks a lot in advance!

 



#2 bob1

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Posted 23 April 2014 - 02:54 PM

It is possible that there could be some esterification going on (usually requires sulphuric acid though).

 

No idea about the telomeres.



#3 mdfenko

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Posted 24 April 2014 - 04:29 AM

more likely to be a problem with evaporation.


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