I am trying to understand how standard curves are developed. We use Polynomial 4 point in a computer program. What I am interested in is how do I check my standards to make sure that they ran correctly. What should a cut off be for CV for each standard? Do I have to worry about my actual standards being vastly different from my expected standard values? Then I use R^2 value to make sure that my data still fits my standard curve.
Edited by Leelab, 19 April 2014 - 05:08 AM.