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Possible to reuse electroporation cuvettes and how?


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6 replies to this topic

#1 saeko

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Posted 05 April 2002 - 04:04 PM

hi, all. I am wondering if it is possible to reuse the cuvettes for electroporation (biorad's) and how(autoclave?). They are expensive, so if I can reuse them, it would be good.
Thanks.

#2 tck1

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Posted 06 April 2002 - 02:33 AM

I often re-use cuvettes, both biorad and equibio, and have found there to be no problems. However you cannot autoclave the lids therefore contamination poses a greater risk than otherwise. Also I have found that arking (sudden flashes that kill the cells) are more frequent. Good luck

#3 helene

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Posted 08 April 2002 - 10:24 PM

yes, you can re-use the cuvettes. For this, you have to wash them 3x with distilled water and 3x with norvanol/alcool and let them resting in norvanol/alcool in a closed (with paper )erlenmeyer for several hours.
After this time, you remove the alcool/norvanol without removing the paper and let them drying in the returned erlemeyer (always closed!!!!) .

  I hope that you've understand my bad english!!
                 
           helene


#4 pengfei

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Posted 28 January 2005 - 07:59 AM

Can't you just wrap the openining of the cuvette with tin foil then autoclave them. Contamination won't be a problem then. Caps are not really needed for electroporation anyway.

#5 tfitzwater

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Posted 31 January 2005 - 11:21 AM

Cuvetttes that have experienced arcing should not be reused.

#6 mikew

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Posted 01 February 2005 - 03:20 PM

I've actually found the efficiency of transfection increases in suspension cells
when I am reusing the cuvettes for the 2nd or 3rd time.
To clean, simply wash in deionized H2O and then soak in ethanol in a tissue culture hood.
Use clean gloves. The cuvettes can also be irradiated by UV in the hood. It works quite well.

#7 fred_33

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Posted 02 February 2005 - 01:51 AM

I reuse mu cuvettes after three wah of distilled water and trhee washes with 70% ethanol (and not the contrary because i've experienced that dna coul be fixed in the cuvette and therefore make contamination) after these washes i put all cuvettes in 50% ethanol for 30minutes and then return them on towel paper and let them to dry. For conservation i use a closed disposal dedicated for them.

I've done 15 electromporations with the sames cuvettes and don't get any problem.

But i always change the cuvette when using a new vector.

Finally, my cuvettes don't resist to an autoclave. Maybe yours? but i don't think so.




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