Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Mean of fluorescence in FlowJo

mean statistics flow cytometry flow jo SYBR

  • Please log in to reply
1 reply to this topic

#1 Judith_NM

Judith_NM

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 18 April 2014 - 04:46 AM

Dear colleagues,

 

I'd like to know how to calculate the mean of fluorescence per cell by flow cytometry.

 

I'm just measuring the total amount of DNA in one wt cyanobacteria spp, and comparing with the total amount of DNA with a modificated strain that is suposed to have more DNA (so I should detect more DNA per cell). I'm using SYBR green.

 

 

So, I just would like to compare the mean of fluorescence (SYBR) per cell in both strains. I just need relative units (for example, if mutant have double amount of DNA than wt).

 

 

 

In Flow Jo there is a tool called "mean". You can apply this statistic to a particular fluorescence (I understand that this means: mean of fluorescence/cell)

 

 

 

Does anyone know if I can just use that measurement for the publication? Or should I normalize this value somehow? Not sure...



#2 JGBarin

JGBarin

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 11 August 2014 - 09:36 AM

Easy rule of thumb.

 

If your population is normally distributed, "Mean" means the arithmetic mean, and assumes a Gaussian distribution.

 

If you're looking at a non-normally distributed population - a population curve that shows asymmetry on histogram, or a pronounced shoulder - use the geometric mean.

 

For more specific detail, you can consult a professional statistician - many larger institutions will have stats consulting core groups. But their tendency will be to overcomplicate your analysis.







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.