I have troubles in quantify dsDNA pre-sequencing in the Picodrop, so I heard about the Qubit, an equipament that quantify dsDNA by the fluorecence, for the use of this equipament we need a kit that has a fluorophore for DNA
I know that the StepONE Plus qpcr has a sensor of fluorescence, so does anybody know if its possible to quantify the dsDNA with this sensor using a kit with a fluorophore for dsDNA? my samples are product of a convencional PCR and to sequencing my samples I need the dna in 4ng/ul.
The Qubit in my country is a little expensive, so if i can use the stepone plus to quantify my dsDNA I will save some important money
Thanks for all
Edited by Felippe Martins, 17 April 2014 - 12:40 PM.