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finding a reference for a formula


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#1 xxcici

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Posted 17 April 2014 - 05:59 AM

where can I find the formula for calculating RNA copy numbers from its ng/ul ? I know the formula, but I could not find the reference. I want to put the reference in my writing, and tried to look for it in some papers and books, but I have not find it. So, where is this formula from? Thanks very much. 



#2 pcrman

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Posted 17 April 2014 - 10:21 AM

The calculation has to be based on the sequence. There is an online tool allowing you to do that http://endmemo.com/bio/dnacopynum.php. maybe you can cite the website.

 

This paper may also help:

Determination of the exact copy numbers of particular mRNAs in a single cell by quantitative real-time RT-PCR



#3 xxcici

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Posted 17 April 2014 - 03:17 PM

The calculation has to be based on the sequence. There is an online tool allowing you to do that http://endmemo.com/bio/dnacopynum.php. maybe you can cite the website.

 

This paper may also help:

Determination of the exact copy numbers of particular mRNAs in a single cell by quantitative real-time RT-PCR

Thank you. I know that web. In the paper, there is no formula:)






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