I'm going to do an IP experiment soon. I will have a duplicate of the sample that will be either IPed with His-SELECT affinity gels or IPed with inactivated gels (for control). My question is what's the best way to inactivate the beads before the IP experiment. I can think of 2 options: boil them for 10minutes or treat them with high concentration of imidazole. Anyone has any suggestion?
Thanks in advance!