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HRP antibody choice

HRP antibody western blot IP

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2 replies to this topic

#1 ulujm

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Posted 16 April 2014 - 09:38 AM

Hi

 

I want to detect a Human protein

 

what second HRP antiboy should I use Mice or Human.

 

Should I use  the 2nd HRP antibody according where the first was produced from (mice or rabbit or goat...) or pick the 2nd HRP antibody according my protein origin (meaning Human)?

 

 

thanks

 

 



#2 Artemis2007

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Posted 16 April 2014 - 12:04 PM

Your secondary needs to be against the species in which the primary antibody was produced. And your primary antibody need to be sensitive to the human protein you're detecting.



#3 ulujm

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Posted 17 April 2014 - 02:30 AM

Thanks.

 

Also I read that people do a control to checkHeavy ad light Chaine. any idea how to do the control?

 

thanks

 

jm







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