2 replies to this topic
Posted 16 June 2004 - 07:26 PM
I had a purified antibody against a synthesized peptide (which conjugated by KLH). Now, I want to purify another peptide, which belong to the same family as the peptide to produce the antibody. Is possible for me to purify another peptide(s) by affinity chromatograph column (i.e.antibody coupled to the agarose gel)? I do not want to purify the peptide by RP-HPLC.
Posted 21 June 2004 - 06:04 PM
You can try but it may not work. Your best bet would be to use an affinity matrix that uses a biological receptor to the peptide; not an antibody. Do you have information on what the biological function of the peptide might be?
Name: Don't Be Cheated