Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

Questions about Paraffin Sectioning

paraffin wax sectioning histology IHC

  • Please log in to reply
No replies to this topic

#1 gsb218



  • Members
  • Pip
  • 1 posts

Posted 15 April 2014 - 07:03 AM

I've been doing some paraffin sectioning, and I've noticed that sometimes after I melt the sections the wax gets this mottled look rather than melting smoothly. Sometimes it is only one or two slides from the same block. I thought it might be because they weren't air dried long enough before melting, and there is still water beneath the sections. However, the last set of blocks I cut were air dried for a few hours before being heated, and still had this problem. I generally air dry the slides on edge while cutting and then melt at 37 deg C overnight. Does anyone have any ideas why this is happening?

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.