I had a question about contamination in the No Template control on the Step One Plus real-time PCR by Life technologies.
I dont want to sound paranoid but I have a feeling that the water contaminations are being caused on purpose. I have never had any problems with the water contamination and all my runs have worked quite well, I have been running tests for a while now and only until recently they have started to get water contamination. I have a feeling that one of my coworkers might have tempared with the Step One Plus template files, so that somehow no matter how I set up my plate I will still get water contamination, even though technically there is no template in the well.
Does anyone have any expereince with the Step One Plus and its software? Is it even feasible to modify a Step One Plus template that you would always get water contamination, and if its not the template file can some other file be changed that would always make the No Template Control show amplififcation.