I am interested to analyze palmitic and 16-hydroxyhesadecanoic acid at the gas chromatograph using DB-WAX column with a FID detector. I have both the standards.
I am doing the esterification of the fatty acids to fatty acid methyl esters (FAMEs) using the alkylation derivatization reagent BCl3-Methanol. Unfortunately the esterification of the 16-hydroxyhesadecanoic can not be achieved. Can someone can give me an explanation?
Furthermore, which kind of derivatization for both the compounds can be used for their analysis at GC-FID (reagents, columns, protocols.…)
Thank you in advance for the help!!!!