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hydroxy-fatty acids and gas chromatography

Gas chromatography fatty acid

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#1 GCrF

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Posted 14 April 2014 - 03:21 AM

Dear all,
I am interested to analyze palmitic and 16-hydroxyhesadecanoic acid at the gas chromatograph using DB-WAX column with a FID detector. I have both the standards. 

I am doing the esterification of the fatty acids to fatty acid methyl esters (FAMEs) using the alkylation derivatization reagent BCl3-Methanol. Unfortunately the esterification of the 16-hydroxyhesadecanoic can not be achieved. Can someone can give me an explanation?

Furthermore, which kind of derivatization for both the compounds can be used for their analysis at GC-FID (reagents, columns, protocols.…)

Thank you in advance for the help!!!!
 


#2 mdfenko

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Posted 14 April 2014 - 03:56 AM

i can't answer your question. in case no one else here can (i've not seen gc questions here), you can ask your question at the chromatography forum.


talent does what it can
genius does what it must
i do what i get paid to do





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