Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

hydroxy-fatty acids and gas chromatography

Gas chromatography fatty acid

  • Please log in to reply
1 reply to this topic

#1 GCrF



  • Members
  • Pip
  • 1 posts

Posted 14 April 2014 - 03:21 AM

Dear all,
I am interested to analyze palmitic and 16-hydroxyhesadecanoic acid at the gas chromatograph using DB-WAX column with a FID detector. I have both the standards. 

I am doing the esterification of the fatty acids to fatty acid methyl esters (FAMEs) using the alkylation derivatization reagent BCl3-Methanol. Unfortunately the esterification of the 16-hydroxyhesadecanoic can not be achieved. Can someone can give me an explanation?

Furthermore, which kind of derivatization for both the compounds can be used for their analysis at GC-FID (reagents, columns, protocols.…)

Thank you in advance for the help!!!!

#2 mdfenko


    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,266 posts

Posted 14 April 2014 - 03:56 AM

i can't answer your question. in case no one else here can (i've not seen gc questions here), you can ask your question at the chromatography forum.

talent does what it can
genius does what it must
i do what i get paid to do

Also tagged with one or more of these keywords: Gas chromatography, fatty acid

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.