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HPLC detection of 5-methylcytidine

HPLC nucleotide detection 5-methylcytidine

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3 replies to this topic

#1 ewright

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Posted 12 April 2014 - 11:50 AM

I have DNA of reducing folate concentration digested with Nuclease P1 hoping to show the differences in 5-methylcytidine for my undergrad senior seminar class.  However, I cannot find a place that sells the standard 5-methylcytidine, and therefore, I have no idea which peak it is or if it is even showing. Any ideas on how I can quantify it?


Edited by ewright, 12 April 2014 - 11:51 AM.


#2 phage434

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Posted 12 April 2014 - 07:26 PM

Many people sell it. Sigma, for instance:

http://www.sigmaaldr...ng=en&region=US



#3 ewright

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Posted 13 April 2014 - 07:20 AM

My bad, I meant 5-methyl-2'deoxycytosine 



#4 phage434

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Posted 13 April 2014 - 08:54 AM

Many people sell it:

http://www.chemicalb...N_CB1132913.htm







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