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how to diminish the background in immunocytochemistry of yh2ax

yh2ax immunocytochemistry

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#1 BioS

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Posted 12 April 2014 - 07:52 AM

Hello

 

I would like to know if someone can tell me how to diminish the background in immunocytochemistry of yh2ax

and which method is better to quantify it.

 

Thanks.



#2 mdfenko

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Posted 14 April 2014 - 03:48 AM

tell us what you have done thus far.

 

usually high background is due to poor blocking and/or (lack of) specificity of an antibody.


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