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Question about IgG & IgM cross reactivity

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#1 betty



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Posted 11 April 2014 - 03:55 AM

I use a standard ELISA looking for antibodies to glycolipds, patients have either IgG or IgM antibodies but it is observed that  a patient with an IgM antibody will cross react with IgG.  This is a false positive. 


How can I stop this cross reaction ?

#2 Tabaluga


    Making glass out of shards

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Posted 13 April 2014 - 11:19 AM

There are methods for eliminating cross-reactions such as described here http://jcm.asm.org/c.../43/4/1944.full but also in this paper it says that none of them proved effective. What did you try ?

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.


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