Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Using pBABE plasmids for regular transfection

pBABE cloning transfection

  • Please log in to reply
1 reply to this topic

#1 yo4trad

yo4trad

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 10 April 2014 - 04:03 AM

Im confused regarding the promoter on the pBABE puro plasmids. Are they usable in regular transfection into mammalian cells with electroporation etc? Do they only work in retroviral transfections. Are the LTR repeats the promoter or the pBABE itself?

 

Thanks



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,597 posts
384
Excellent

Posted 10 April 2014 - 12:05 PM

IIRC they should work for normal transfection.  I don't know for certain but I suspect the LTRs are for the recombination events required to make the retroviral genome.







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.