I have isolated bacteria from tungsten and is looking forward for its use in bioremediation. I have send the bacteria samples for ICPMS, to check out for tungsten accumulation. I would like to know if there any particular protein responsible for metal uptake?? Also tungsten acts as a cofactor for many of the enzymes in bacteria. So if i isolate the total protein of a bacteria, is it possible to detect the metal in the protein which binds as a cofactor by any means?
detection of metal in protein
Posted 11 April 2014 - 04:37 AM
genius does what it must
i do what i get paid to do
Posted 11 April 2014 - 06:02 AM
Is it possible to check the metal concentration in the protein by ICP-MS?
Also what amount of metal could be predicted as bound to the protein?
Further can you please elaborate on how should I proceed with the experiment?