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detection of metal in protein


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4 replies to this topic

#1 Hiral

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Posted 10 April 2014 - 03:06 AM

I have isolated bacteria from tungsten and is looking forward for its use in bioremediation. I have send the bacteria samples for ICPMS, to check out for tungsten accumulation. I would like to know if there any particular protein responsible for metal uptake?? Also tungsten acts as a cofactor for many of the enzymes in bacteria. So if i isolate the total protein of a bacteria, is it possible to detect the metal in the protein which binds as a cofactor by any means?



#2 bob1

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Posted 10 April 2014 - 12:06 PM

Sure - you could do some FAAS.



#3 Hiral

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Posted 10 April 2014 - 07:45 PM

What does FAAS stand for, and how do I go about it?



#4 mdfenko

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Posted 11 April 2014 - 04:37 AM

flame atomic absorption spectrometry
talent does what it can
genius does what it must
i do what i get paid to do

#5 Hiral

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Posted 11 April 2014 - 06:02 AM

Is it possible to check the metal concentration in the protein by ICP-MS?

Also what amount of metal could be predicted as bound to the protein?

Further can you please elaborate on how should I proceed with the experiment?






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