Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Ionizing radiation dose for gene expression profiling

cancer radiobiology gene expression radio oncology dna damage & repair

  • Please log in to reply
No replies to this topic

#1 sen111



  • Active Members
  • Pip
  • 12 posts

Posted 09 April 2014 - 07:08 AM

           I am planning to do a real-time PCR array based differential gene expression profiling of DNA repair genes. First I am going to knockout gene of our interest (GOI) followed by irradiation (cs 137, gamma radiation) & RNA isolation (24 hrs post irradiation).

I have a problem in choosing the radiation dose. 

1).What dose can I use to see a difference in gene expression? Say 5Gy or 10Gy
2).What is the rationale in choosing the dose.

Thanks in advance

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.