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Ionizing radiation dose for gene expression profiling

cancer radiobiology gene expression radio oncology dna damage & repair

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#1 sen111

sen111

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Posted 09 April 2014 - 07:08 AM

Hello,
           I am planning to do a real-time PCR array based differential gene expression profiling of DNA repair genes. First I am going to knockout gene of our interest (GOI) followed by irradiation (cs 137, gamma radiation) & RNA isolation (24 hrs post irradiation).

I have a problem in choosing the radiation dose. 

1).What dose can I use to see a difference in gene expression? Say 5Gy or 10Gy
2).What is the rationale in choosing the dose.

Thanks in advance
Sen






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