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Cytochalasin use in stimulation assays


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#1 SagoJ

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Posted 09 April 2014 - 06:50 AM

I and many others have used Cytochalasin B in stimulation assays of neutrophils, in for example ROS and degranulation assays. If I understand this correctly it is to enhance the signal. Does anyone know how? I read that CyB inhibits actin filaments and glucose uptake, but this does not make sense in the ROS and degranulation experiments, or?

 



#2 Thom

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Posted 11 June 2014 - 11:58 AM

Maybe this can help:

http://www.biomedcen...1-2121-5-21.pdf






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