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Protein Purification from mammalian cells

Purification mammalian

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#1 Andy1

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Posted 08 April 2014 - 10:47 PM

Hi, as the title says my boss told me to purify proteins from mammalian cells using ammonium sulfate fractionalization and ion exchange chromatography. Can you please provide some guidelines for ion exchange chromatography for proteins from mammalian cells, how much proteins should I have and in what volume for this to be economically viable, where are the pitfalls, how much time will it take.



#2 mdfenko

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Posted 09 April 2014 - 04:06 AM

this handbook and tip sheet should help:

 

 

 

and you should look at these handbooks from ge lifesciences.

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Edited by mdfenko, 09 April 2014 - 04:07 AM.

talent does what it can
genius does what it must
i do what i get paid to do




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