Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Absorption characteristics of proteins

protein yellow cofactor spectrum

  • Please log in to reply
No replies to this topic

#1 Ronny28

Ronny28

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 08 April 2014 - 06:43 AM

Hi everybody,

 

I have some questions about the absorption of proteins. First a general question, why have most proteins a yellow color at high concentrations, even though they do not bind a cofactor? What causes this absorbtion?

 

I work with a protein which is slightly yellow at low concentrations (1mg/mL). I run a UV-vis scan which showed a weak absorption shoulder at 420-30nm. FeS cluster binding proteins show the same weak shoulder at 420nm. But a sequence analysis gave no hint of the presence of a typical Fe or FeS binding motif.  

Could this high-concentration-yellow give rise to a shoulder at 420? There are no homologs, maybe there are some similarities to NADH binding proteins, but with bad score. Could the absorption of NADH shift to 420nm when bound to a protein?

Could other cofactors or metals be present? I don´t think that PLP or FAD are present because the absorption spectra look different.

 

I would be glad for some advise,

 

Ronny

Fixed your size problem... Bob.







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.