I want to test the metabolic toxicity for renal cell.
I have searched the protocol for how to use S9 fraction.
In the protocol. After i treat drug with S9 fot 3~4 hrs, I should to aspirate it off and wash with PBS.
But the renal cell that i used is HEK293, it is weak adhesion on well-plate.
How can i do to prevent to wash down cells?