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metabolic toxicity for non-attached cells

S9 fraction

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#1 cell 293

cell 293

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Posted 08 April 2014 - 01:27 AM

Hi

 

I want to test the metabolic toxicity for renal cell.

 

I have searched the protocol for how to use S9 fraction.

 

In the protocol. After i treat drug with S9 fot 3~4 hrs, I should to aspirate it off and wash with PBS.

 

But the renal cell that i used is HEK293, it is weak adhesion on well-plate.

 

How can i do to prevent to wash down cells?

 

Thanks






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