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What sencondary for Chimera Fc primary antibody?

Chimera Fc Secondary Ab WB IHC Mouse myeloma cell line

Best Answer tkf, 08 April 2014 - 10:38 AM

According to the data sheet, you should use anti-Human IgG secondary Ab.

 

http://www.pierce-an...yAntibodies.cfm

 

Also, your RnD product is not an antibody, its a fusion between your protein (ICAM) and Fc of Human IgG.

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#1 braincell

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Posted 07 April 2014 - 08:34 AM

Hi all, 

 

I got hold of a Chimera Fc primary antibody that I would like to use it for WB and IHC. However I can't figure out what sencondary I should use. The Data Sheet says the antibody was produced in a mouse myeloma cell line (NS0-derived). None of the citated papers used it for WB or IHC.

 

Any suggestion?

 

Thanks!



#2 tkf

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Posted 07 April 2014 - 10:29 AM

You probably need an anti-mouse IgG secondary Ab.

Could you upload that data sheet?



#3 braincell

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Posted 07 April 2014 - 12:23 PM

Thanks tkf!

 

here the data sheet      http://www.rndsystem.../pdf/796-ic.pdf



#4 tkf

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Posted 08 April 2014 - 10:38 AM   Best Answer

According to the data sheet, you should use anti-Human IgG secondary Ab.

 

http://www.pierce-an...yAntibodies.cfm

 

Also, your RnD product is not an antibody, its a fusion between your protein (ICAM) and Fc of Human IgG.


Edited by tkf, 08 April 2014 - 10:41 AM.


#5 braincell

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Posted 08 April 2014 - 11:10 AM

I see... now everything makes sense. So it is not an antibody-antigen situation but a ligand-receptor.

 

Thanks you so much tkf!







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