Let's say you have an antigen which you've confirmed, by ELISpot, that it is immunogenic in mice. How would you go about identifying the T cell receptor(s) that are responding to the antigen?
Submit your paper to J Biol Methods today!
How to identify CD8 T cell receptor for a particular antigen
Started by Ahrenhase, Apr 06 2014 11:25 PM
2 replies to this topic
#1
Ahrenhase
-
- Active Members
-
- 178 posts
Veteran
11
Good
Good
#2
tkf
-
- Active Members
-
- 33 posts
Enthusiast
1
Neutral
Neutral
Posted 07 April 2014 - 10:39 AM
Step 1: Isolate the Abs to your antigen.
Step 2: Generate new Abs against the variable regions (CDRs) of these Abs.
Hypothetically, the Abs from step 2 should recognize any receptors to which your antigen binds.
- Ahrenhase likes this
#3
Ahrenhase
-
- Active Members
-
- 178 posts
Veteran
11
Good
Good
Posted 09 April 2014 - 06:04 AM
Step 1: Isolate the Abs to your antigen.
Step 2: Generate new Abs against the variable regions (CDRs) of these Abs.
Hypothetically, the Abs from step 2 should recognize any receptors to which your antigen binds.
Interesting, thanks.
