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How to identify CD8 T cell receptor for a particular antigen


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#1 Ahrenhase

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Posted 06 April 2014 - 11:25 PM

Let's say you have an antigen which you've confirmed, by ELISpot, that it is immunogenic in mice.  How would you go about identifying the T cell receptor(s) that are responding to the antigen?



#2 tkf

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Posted 07 April 2014 - 10:39 AM

Step 1: Isolate the Abs to your antigen.

Step 2: Generate new Abs against the variable regions (CDRs) of these Abs.

Hypothetically, the Abs from step 2 should recognize any receptors to which your antigen binds.



#3 Ahrenhase

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Posted 09 April 2014 - 06:04 AM

Step 1: Isolate the Abs to your antigen.

Step 2: Generate new Abs against the variable regions (CDRs) of these Abs.

Hypothetically, the Abs from step 2 should recognize any receptors to which your antigen binds.

 

Interesting, thanks.  






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