Let's say you have an antigen which you've confirmed, by ELISpot, that it is immunogenic in mice. How would you go about identifying the T cell receptor(s) that are responding to the antigen?
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How to identify CD8 T cell receptor for a particular antigen
Started by Ahrenhase, Apr 06 2014 11:25 PM
2 replies to this topic
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Ahrenhase
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tkf
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Posted 07 April 2014 - 10:39 AM
Step 1: Isolate the Abs to your antigen.
Step 2: Generate new Abs against the variable regions (CDRs) of these Abs.
Hypothetically, the Abs from step 2 should recognize any receptors to which your antigen binds.
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Ahrenhase
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Posted 09 April 2014 - 06:04 AM
Step 1: Isolate the Abs to your antigen.
Step 2: Generate new Abs against the variable regions (CDRs) of these Abs.
Hypothetically, the Abs from step 2 should recognize any receptors to which your antigen binds.
Interesting, thanks.
