Let's say you have an antigen which you've confirmed, by ELISpot, that it is immunogenic in mice. How would you go about identifying the T cell receptor(s) that are responding to the antigen?
How to identify CD8 T cell receptor for a particular antigen
Posted 09 April 2014 - 06:04 AM
Step 1: Isolate the Abs to your antigen.
Step 2: Generate new Abs against the variable regions (CDRs) of these Abs.
Hypothetically, the Abs from step 2 should recognize any receptors to which your antigen binds.