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fixation and methylation

fixation methylation epigenetics

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2 replies to this topic

#1 neuron00

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Posted 06 April 2014 - 10:59 AM

I am planning on extracting DNA from tissues for a methylation study.  Would a prior methanol fixation affect the DNA extraction or methylation?  Thanks



#2 phage434

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Posted 06 April 2014 - 06:43 PM

I doubt if this will be a problem.



#3 pcrman

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Posted 08 April 2014 - 09:03 AM

Nope. But keep in mind, since DNA from paraffin blocks is already degraded, and will be further fragmented during bisulfite conversion, amplicons size should be kept to a minimum when designing primers. 







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