We have modified the current DNA cloning method. Our modified method is especially suitable for the challenging cloning (e.g., blunt-end DNA cloning, single-digested DNA cloning and some challenging double-digested DNA cloning). The modified method is simple and convient. We think it worth a try if you have some trouble during molecular cloning.
If it is helpful, please do not forget to cite it, thank you before hand. Good luck for your work!!!