Hi, I'm facing problem in counting my Aspergillus Niger.
I grew the Aspergillus Niger on Sabouraud dextrose agar with chloramphenicol(SDAC), incubated for 5 days. Then I washed the plate with 9ml of 0.001% Tween 80, this I considered as 108 suspension.
Then I diluted to 102 suspension and took 0.1ml to do spread plate on SDAC, incubated for 5 days.
The problem is after 5 days incubation, my Aspergillus grows all over the agar surface, I cannot get separate colonies.
May I know what is the proper way to count the Aspergillus? I need to know the viable count for my 102 suspension.