Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

What is the best statisical analysis to use?

Statisics Anova Cell Assays

  • Please log in to reply
1 reply to this topic

#1 loud



  • Members
  • Pip
  • 1 posts

Posted 04 April 2014 - 10:16 AM



I ran an experiment to measure the effect of two drugs combined on cell apoptosis.  I used 8 different concentrations of the first  drug alone, and 8 different concentrations of the first drug combined with a constant concentration of the second.  I measured cell viability using the MMT method.


Which ANOVA analysis would be best to use?  I thought two way as I wanted to measure the effect at each concentration and compare it to each concentration of the first drug alone.


It was suggested to me to use one way as I am only measuring one factor, but I consider it to be two. 


Any help would be appreciated.

#2 DRT



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 176 posts

Posted 07 April 2014 - 12:17 AM

What you are looking to compare is two dose response curves so ideally you would look to compare the (logistic) parameters that describe that response; but the estimation of the errors surrounding non-linear parameters can be tricky. A graph with error bars at each concentration may be all the evidence you need.


As a fall back, you have designed the trial as a form of paired test at each of the 8 concentrations of the first drug, so it is possible to test the significance of the difference between the presence or absence of the second drug. Given the non-linearity perhaps the best start point is a non-parametric test.


As a final comment, be careful not to get caught into claims of synergy. At the very least you need a dose response for the second drug on its own.


good luck

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.