Which approach is the best for purification of a sample for TOPO?
a) run a small PCR-sample on electrophoresis (5ul). If I get a clear band I will then purify the other PCR-sample (45ul) that I did not run on agaros electrophoresis
b ) run 2 lanes of electrophoresis, one lane with 5ul and one with 45ul. The one with 5ul I expose to UV to see whether I get a clear band, and if I do then I purify the PCR-product from the other agaros-electrophoresis (which I save from UV-exposure).
I am thinking that a) is a more preferable approach since purification from a solution would give BETTER YIELD than purification from agaros? Any comments on this?