Extracting RNA from Staphylococcus epidermidis using Qiagen RNeasy kit, then I DNase the samples using promega DNase and do qRT-pcr....but gDNA still present.
When i have tried qiagen DNase and Turbo DNase in different experments, gDNA still presents also.
I don't know what to do to get ride of gDNA....if staphylococcus DNA is so hard to get ride of????
Thanks in advance.
Edited by Shahira, 03 April 2014 - 04:26 PM.