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Heat the sample during nitrogen evaporation of peptides eluted in ethanol/6 N hy

chromatography Nitrogen Evaporation Proteins

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#1 Nicolai

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Posted 03 April 2014 - 11:04 AM

I am doing nitrogen evaporation of a sample of proteins eluted from a sep-pak carteridge in ethanol/6 N hydrochloric acid (1,000:1, v/v). The protein of interest is Oxytocin.

My question is: should I use heat the sample while evaporating? I would think that heating would cause more rapid evaporation, but cold temperature would be safer for protein integrity.. However, will the EtOH/HCL solvent evaporate efficiently without heat??

Any thoughts and comments are greatly appreciated. 



#2 mdfenko

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Posted 21 April 2014 - 03:40 AM

oxytocin should be reasonably stable with gentle heating (37C). keep in mind that evaporation will chill the sample so you could also heat to 25C.


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#3 Nicolai

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Posted 23 April 2014 - 01:21 PM

Hello mdfenko,

 

Thank you for your thoughts on the topic, greatly appreciated!

 

Nicolai







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