We are having some troubles with our FACSCanto II cytometer: it is not showing any event in wells where we positively know there's a big amount of cells. We performed several primes to solve possible needle obstructions, followed by a long clean and all the possible cleaning modes existing in the machine. We also filled the fluidics filters, checked the waste tanks and cubitainers etc. we did everything we thought of, but still any event was being detected (no acquisition light was blinking). The lasers were checked about a month ago and we don't really think it is a laser problem...
Any idea where is the problem? We are going mad!
Thanks a lot