I am new in this cloning world and I need to design primers for cloning. In general I know that the forward primer must seem something like following:
FLANKING-Restriction site-Kozak-Start Codon-gene of interest-Stop Codon-FLANKING
Now I am wondering how should I design my reverse primer?
I would really appreciate any help and tips to primers designing for cloning
Edited by Cell buster, 03 April 2014 - 07:20 AM.