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Ampicillin as positive control

ampicillin concentration

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#1 Dima

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Posted 03 April 2014 - 05:26 AM

Hello!

 

can anyone suggest why the ampicillin when used as a positive control didnt make a change against bacteria such as pseudo.aerog. and others like listeria? i used it in 48 well plate with broth culture, not agar plate. so i was applying it at RT and there is no hot media or agar that may have degraded the product.

 

I used 0.2 mg/1ml

 

do i need to increase the concentration? any reference or guidelines are available and could be helpful on the optimum usage of ampicillin salt??

 

Always greatly appreciated your help!!



#2 Phil Geis

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Posted 03 April 2014 - 06:11 AM

Pseudomonas aeruginosa is resistant to ampicillin, and this antibiotic is only bacteriostatic vs. Listeria.



#3 Dima

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Posted 03 April 2014 - 07:01 AM

what is the positive control i should use against those bac?

 

i have five bacteria: staph aureus, e.coli, listeria monocytogenes, salmonella and pseudomons.

 

In that case i need to use different antibiotic that act on each specifically?



#4 Phil Geis

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Posted 03 April 2014 - 09:00 AM

What are you trying to accomplish - your hypothesis?






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