I have Nanodrop reading and need help knowing if DNA is pure or not. I need a second opinion.
The 260/280 reading is 2.17. I have seen places say 1.8 is ideal for DNA 260/280 purity. I have seen some other places go from 1.8-2.0. I believe that 2.17, there could be possible protein contaminants.
The 260/230 is 3.15. Which is higher than the normal 2.0-2.2 range. I read the DNA in triplicates, and the lowest of the triplicates for 260/230 was 2.65. Does it mean it there is a lot of chemical contaminants? What type of chemical will give a reading like this, between 2.65-3.15? I blanked with water and DNA buffer, then measured each as negative controls. They all read 0, so they are both at baseline.