i'm doing a report for a biochemistry lab where we performed TLC. for the mobile phase we used a mixture of isopropyl, water and amonia (in a ratio of 8:1:1, 8 being the isopropyl). I thought that this mixture is hydrophilic, since all of it's components are, but my tutor/instructor says that isopropyl is extremely hydrophobic, and so the mixture is also hydrophobic. i googled it and from what i saw IT IS considered hydrophilic.
so, if you can tell me what's right, and why, i'll be grateful:)
Edited by liika, 02 April 2014 - 08:40 AM.