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Can I use cellufine sulfate cloumn as a strong cation exchange?


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#1 SHODAN

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Posted 02 April 2014 - 04:31 AM

Hi everyone,

 

I need a strong cation exchange to purify my protein, and we don't have any. I got a cellufine sulfate cloumn, it's with sulfate ester group. I read that the use of the column is specific for viruses and heparin binding proteins, so it's considered as a affinity column.
Can i still use it as a  strong cation exchange? The binding and elution buffers are similar to a IEX chromatography. 


Edited by SHODAN, 02 April 2014 - 04:39 AM.


#2 bob1

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Posted 02 April 2014 - 11:57 AM

It's structure doesn't look like an ion exchange polymer to me, but I'm no expert



#3 mdfenko

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Posted 03 April 2014 - 04:48 AM

based on what i read in the technical data sheet (pdf) for cellufine sulfate, you may be able to use it for ion exchange but it seems to exhibit selective binding so it depends on whether or not your protein(s) of interest binds.

 

you can try it (save everything).


Edited by mdfenko, 03 April 2014 - 04:50 AM.

talent does what it can
genius does what it must
i do what i get paid to do




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