I need a strong cation exchange to purify my protein, and we don't have any. I got a cellufine sulfate cloumn, it's with sulfate ester group. I read that the use of the column is specific for viruses and heparin binding proteins, so it's considered as a affinity column.
Can i still use it as a strong cation exchange? The binding and elution buffers are similar to a IEX chromatography.
Edited by SHODAN, 02 April 2014 - 04:39 AM.