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Non-denaturing buffer to extract nuclear protein


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#1 chimera

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Posted 02 April 2014 - 03:01 AM

Can anybody suggest me a non-denaturing buffer composition for extracting nuclear protein? I will be using that protein for the purpose of isolating a multi-protein complex. Will inclusion of 110 mM KCl in the lysis buffer disrupt the protein -protein interaction among the subunits? Please help me. Thanks for your patience.



#2 bob1

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Posted 02 April 2014 - 11:58 AM

The key here is probably detergents - avoid SDS containing buffers, triton will pop the nuclear membrane.  You could also look at mechanical disruption.






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