Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

DOSE RPMI 1640 with additives more better than without?


  • Please log in to reply
1 reply to this topic

#1 semsem shaltout

semsem shaltout

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 31 March 2014 - 03:21 PM

DOSE RPMI 1640 WITH l glutamine and HEPES and NA pyruvate and high glucose more better on cell culture than with l-glutamine only?i haven't started my research yet, if i use this type of media from the start will improve the quality and count of isolated PBMCS FROM BLOOD????or the result not differ from media with only L- GLUTAMINE


Edited by semsem shaltout, 31 March 2014 - 10:43 PM.


#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,784 posts
405
Excellent

Posted 31 March 2014 - 07:21 PM

It depends on the cells!  If you are already growing them in RPMI with only L-glut as a supplement, then you should continue with that unless you have a very good reason not to.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.