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DOSE RPMI 1640 with additives more better than without?

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#1 semsem shaltout

semsem shaltout


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Posted 31 March 2014 - 03:21 PM

DOSE RPMI 1640 WITH l glutamine and HEPES and NA pyruvate and high glucose more better on cell culture than with l-glutamine only?i haven't started my research yet, if i use this type of media from the start will improve the quality and count of isolated PBMCS FROM BLOOD????or the result not differ from media with only L- GLUTAMINE

Edited by semsem shaltout, 31 March 2014 - 10:43 PM.

#2 bob1


    Thelymitra pulchella

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Posted 31 March 2014 - 07:21 PM

It depends on the cells!  If you are already growing them in RPMI with only L-glut as a supplement, then you should continue with that unless you have a very good reason not to.

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