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Dna isolation from fungi

Dna extraction fungi

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#1 Shivani1

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Posted 31 March 2014 - 09:57 AM

Hello,

 

I am trying to extract Dna from fungus (Alternaria alternata). I need really high quality dna for southern blots. Till now, i have tried a variety of methods, from CTAB method to qiagen kits. But, all i get is degraded dna or very low concentrations that are not usable at all. I always get a nice pellet but when i run the dna on a gel, it shows up as a smear.

 

Please help.

 

Thanks.

 

 



#2 pito

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Posted 31 March 2014 - 10:09 AM

you have a picture of your gels?

Its hard to say how bad/good your results are to be honest and: smears are in general not that that weird to have when doing a genomic DNA extraction.


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#3 Shivani1

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Posted 31 March 2014 - 11:43 AM

Here's a gel picture. The first two samples showed a huge Rna cloud, so i made sure to do a Rnase treatment after phase separation with chloroform. But then, the three samples showed only a smear. There's barely any dna in there even though my nanodrop showed a concentration of around ~500ng/ul.

 

Thanks.

 

gel.png







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