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LB media contamination


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#1 Austinys

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Posted 31 March 2014 - 07:43 AM

Hi,

 

I autoclaved my LB media in 500 ml flask with foil cap, but I will start my experiment just next week. I can't hold flask in refrigerator, due to lack of space. So my question is, could LB media be storaged in room temperature for a week and do not be contaminated?



#2 lmh327

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Posted 31 March 2014 - 07:54 AM

If it is contaminant free (it should be if you autoclaved it) and in a sealed container (even a flask with foil) it will be fine at RT.  We have used our months later.



#3 Austinys

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Posted 31 March 2014 - 09:13 AM

That's good news, thanks!


Edited by Austinys, 31 March 2014 - 09:13 AM.


#4 pito

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Posted 31 March 2014 - 10:20 AM

Storage of media in the fridge is actually only (in most cases) needed if you have added "something" extra (eg antibiotics) or to keep it away from light (but I assume you will not put your bottles right at the window, in the direct sun).

Or for plates (to reduce the loss of moist)

 

A good website for general information: www.cabri.org/guidelines/micro-organisms/M203Ap1.html


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