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purpose of 65 degrees incubation overnight after beads elution for CHIP


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#1 soymilk14

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Posted 30 March 2014 - 06:22 PM

hello guys,

 

i am new in performing CHIP assay, i want to ask a simple question on the purpose of incubating the sample after beads elution at 65 degrees overnight.

 

also, what universal primer can i use just  to check if i have finally eluted my dna and captured my dna fragments by my antibody? or should i simple run a small amount of my clean eluted dna in an agarose gel?

 

thank you..



#2 pcrman

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Posted 08 April 2014 - 09:26 AM

that is to reverse the crosslink done by formaldehyde at the beginning. 






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