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If MCF-7 cell line can expresses Her-2 gene?

Her-2 MCF-7

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#1 michaeltaozmkm

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Posted 30 March 2014 - 12:14 AM

Hi all,

 

I wanna know if MCF-7 cell (the only mammary gland cancer cell I have) can expresses Her-2 gene? Or only SKBR-3 cell can expresses it?

 

Thanks for u help!

 

MT



#2 bob1

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Posted 30 March 2014 - 12:43 AM

MCF-7 don't express Her2 natively, but you could put it on a plasmid and transfect it in.



#3 michaeltaozmkm

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Posted 30 March 2014 - 01:03 AM

MCF-7 don't express Her2 natively, but you could put it on a plasmid and transfect it in.

Thank you, My thinking is if any mammary gland cancer cells can express Her2, I can isolate mRNA, RT-PCR the cDNA and linked with plasmid manually. Because it will wait for about one month if i buy one from Addgene, And that's too long.



#4 bob1

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Posted 30 March 2014 - 01:45 PM

If the line doesn't express the protein, then it is quite likely that they won't produce the mRNA - so you had better get a line that does express so as to be able to get the right gene out.

 

Cloning may well take you more than a month.



#5 michaeltaozmkm

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Posted 30 March 2014 - 09:46 PM

If the line doesn't express the protein, then it is quite likely that they won't produce the mRNA - so you had better get a line that does express so as to be able to get the right gene out.

 

Cloning may well take you more than a month.

Thank you, one more question, do you know if MDA-MB-453 cell line can express Her2? Because I just found I can buy this one here in Shanghai.



#6 bob1

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Posted 31 March 2014 - 12:13 AM

here: http://lmgtfy.com/?q=MDA-MB-453+her2







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