As title states, how can I improve the yield?
I go from 300ng/ul down to barely 20ng/ul.
I tried to incubate longer time at RT (too evaporate the ethanol from the washing step, but at the same time not too long to risk getting over-dried). Also tried to centrifuge at higher speed and for 2min. And to divide my elution down to 2steps (first 20ul and then 10ul).
Not much improvement so far.
I am using Ge Healthcare Illustra Agaros Purification Kit.