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using DMSO and Ethanol at the same time

cell culture

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#1 murusa

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Posted 26 March 2014 - 11:24 PM

hello everyone!

Anyone tried to use DMSO and Ethanol as solvents at the same culture media? in my case I have to use different supplements for media and one of them should be dissolved in ethanol, other ones - in dmso.

is it safe for cells to use both solvents at the same time?

 

thank you in advance!


Edited by murusa, 26 March 2014 - 11:53 PM.


#2 Curtis

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Posted 26 March 2014 - 11:34 PM

what are the supplements?



#3 murusa

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Posted 26 March 2014 - 11:42 PM

dexamethasone, IBMX, Rositaglitazone.

Sigma recommends to dissolve first one in ethanol and then in media.. other drugs should be dissolved in dmso 



#4 bob1

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Posted 27 March 2014 - 01:32 AM

I'm not 100% sure but I think it will be safe - it would probably pay to run a test beforehand just using the solvents at the levels you would if you had added the drugs, to see if you can see any effect on your cells.







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