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Methylation status analysis of non CpG sites.


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4 replies to this topic

#1 Bedanta Ballav Mohanty

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Posted 26 March 2014 - 07:51 PM

Hi.

I want to know how we can check the non CpG methylation status of a gene. Can MSP be used for this purpose?



#2 phage434

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Posted 05 April 2014 - 12:00 PM

Yes. It will pick up any methylated cytosine.



#3 pcrman

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Posted 08 April 2014 - 09:32 AM

it is tricky to use MSP to check non-cpg C methylation since there is not many place you can put your primers in without including a non-cpg C. 



#4 phage434

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Posted 08 April 2014 - 09:45 AM

You can use primers that are degenerate (have a Y instead of a C or T, and an R instead of a G or A).



#5 Robert

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Posted 13 May 2014 - 05:34 AM

Hi

If a software for designing MSP or BSP primers shows: "lack of CpG islands in the promoter" of studied gene, how can be determined methylation of such gene ?






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