I´m new to flow cytometry and are setting up a protocol for flow cytometry on mononuclear cells from mouse blood, but would like some input from people with experience.
I have bought a kit from Cedarlane (Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid) to isolate the mononuclear cells. But I still need some input like:
How much blood is needed to get how much separated cells ?? How many mice are needed ?? How do I collect the blood ??
I´m in need of a protocol for collection of blood and staining of isolated cells.