Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Protocol for flow cytometry on mononuclear cells from mouse blood

Flow cytometry mouse

  • Please log in to reply
No replies to this topic

#1 esbena

esbena

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 26 March 2014 - 03:49 AM

Hallo all.
 
I´m new to flow cytometry and are setting up a protocol for flow cytometry on mononuclear cells from mouse blood, but would like some input from people with experience.
 
I have bought a kit from Cedarlane (Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid) to isolate the mononuclear cells. But I still need some input like: 
 
How much blood is needed to get how much separated cells ?? How many mice are needed ?? How do I collect the blood ?? 
 
I´m in need of a protocol for collection of blood and staining of isolated cells.
 
Thanks






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.