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Protocol for flow cytometry on mononuclear cells from mouse blood

Flow cytometry mouse

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#1 esbena



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Posted 26 March 2014 - 03:49 AM

Hallo all.
I´m new to flow cytometry and are setting up a protocol for flow cytometry on mononuclear cells from mouse blood, but would like some input from people with experience.
I have bought a kit from Cedarlane (Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid) to isolate the mononuclear cells. But I still need some input like: 
How much blood is needed to get how much separated cells ?? How many mice are needed ?? How do I collect the blood ?? 
I´m in need of a protocol for collection of blood and staining of isolated cells.

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